SrasV1, Main, Exploration, bibRecord, 003493

Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2

Identifieur interne : 003493 ( Main/Exploration ); précédent : 003492; suivant : 003494

Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2

Auteurs : Alison C. Mathewson [Royaume-Uni] ; Alexandra Bishop [Royaume-Uni] ; YONGXIU YAO [Royaume-Uni] ; Fred Kemp [Royaume-Uni] ; JUNYUAN REN [Royaume-Uni] ; HONGYING CHEN [Royaume-Uni] ; XIAODONG XU [Royaume-Uni] ; Ben Berkhout [Pays-Bas] ; Lia Van Der Hoek [Pays-Bas] ; Ian M. Jones [Royaume-Uni]

Source :

Journal of general virology [ 0022-1317 ] ; 2008.

RBID : Pascal:08-0506391

Descripteurs français

KwdFr :
- Cinétique, Coronavirus (physiologie), Cytométrie en flux, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Humains, Indice de gravité médicale, Infections à coronavirus (physiopathologie), Liaison aux protéines, Peptidyl-Dipeptidase A (métabolisme), Protéines de l'enveloppe virale (métabolisme), Solutions, Syndrome respiratoire aigu sévère (physiopathologie), Virus du SRAS (physiologie).
MESH :
- métabolisme : Glycoprotéines membranaires, Peptidyl-Dipeptidase A, Protéines de l'enveloppe virale.
- physiologie : Coronavirus, Virus du SRAS.
- physiopathologie : Infections à coronavirus, Syndrome respiratoire aigu sévère.
Pascal (Inist)
- Cinétique, Coronavirus, Cytométrie en flux, Glycoprotéine de spicule des coronavirus, Humains, Indice de gravité médicale, Interaction, Liaison aux protéines, Protéine, Microbiologie, Solutions, Syndrome respiratoire aigu sévère, Angiotensin converting enzyme 2.

English descriptors

KwdEn :
- Coronavirus, Coronavirus (physiology), Coronavirus Infections (physiopathology), Flow Cytometry, Humans, Interaction, Kinetics, Membrane Glycoproteins (metabolism), Microbiology, Peptidyl-Dipeptidase A (metabolism), Protein, Protein Binding, SARS Virus (physiology), Severe Acute Respiratory Syndrome (physiopathology), Severe acute respiratory syndrome, Severity of Illness Index, Solutions, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (metabolism).
MESH :
- chemical , metabolism : Membrane Glycoproteins, Peptidyl-Dipeptidase A, Viral Envelope Proteins.
- physiology : Coronavirus, SARS Virus.
- physiopathology : Coronavirus Infections, Severe Acute Respiratory Syndrome.
- Flow Cytometry, Humans, Kinetics, Protein Binding, Severity of Illness Index, Solutions, Spike Glycoprotein, Coronavirus.

Abstract

Although in different groups, the coronaviruses severe acute respiratory syndrome-coronavirus (SARS-CoV) and NL63 use the same receptor, angiotensin converting enzyme (ACE)-2, for entry into the host cell. Despite this common receptor, the consequence of entry is very different; severe respiratory distress in the case of SARS-CoV but frequently only a mild respiratory infection for NL63. Using a wholly recombinant system, we have investigated the ability of each virus receptor-binding protein, spike or S protein, to bind to ACE-2 in solution and on the cell surface. In both assays, we find that the NL63 S protein has a weaker interaction with ACE-2 than the SARS-CoV S protein, particularly in solution binding, but the residues required for contact are similar. We also confirm that the ACE-2-binding site of NL63 S lies between residues 190 and 739. A lower-affinity interaction with ACE-2 might partly explain the different pathological consequences of infection by SARS-CoV and NL63.

Affiliations:

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Coronavirus (physiology)</term>
<term>Coronavirus Infections (physiopathology)</term>
<term>Flow Cytometry</term>
<term>Humans</term>
<term>Interaction</term>
<term>Kinetics</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Microbiology</term>
<term>Peptidyl-Dipeptidase A (metabolism)</term>
<term>Protein</term>
<term>Protein Binding</term>
<term>SARS Virus (physiology)</term>
<term>Severe Acute Respiratory Syndrome (physiopathology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Severity of Illness Index</term>
<term>Solutions</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Cinétique</term>
<term>Coronavirus (physiologie)</term>
<term>Cytométrie en flux</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Humains</term>
<term>Indice de gravité médicale</term>
<term>Infections à coronavirus (physiopathologie)</term>
<term>Liaison aux protéines</term>
<term>Peptidyl-Dipeptidase A (métabolisme)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Solutions</term>
<term>Syndrome respiratoire aigu sévère (physiopathologie)</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Protéines de l'enveloppe virale</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Flow Cytometry</term>
<term>Humans</term>
<term>Kinetics</term>
<term>Protein Binding</term>
<term>Severity of Illness Index</term>
<term>Solutions</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Cinétique</term>
<term>Coronavirus</term>
<term>Cytométrie en flux</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Indice de gravité médicale</term>
<term>Interaction</term>
<term>Liaison aux protéines</term>
<term>Protéine</term>
<term>Microbiologie</term>
<term>Solutions</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Angiotensin converting enzyme 2</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Although in different groups, the coronaviruses severe acute respiratory syndrome-coronavirus (SARS-CoV) and NL63 use the same receptor, angiotensin converting enzyme (ACE)-2, for entry into the host cell. Despite this common receptor, the consequence of entry is very different; severe respiratory distress in the case of SARS-CoV but frequently only a mild respiratory infection for NL63. Using a wholly recombinant system, we have investigated the ability of each virus receptor-binding protein, spike or S protein, to bind to ACE-2 in solution and on the cell surface. In both assays, we find that the NL63 S protein has a weaker interaction with ACE-2 than the SARS-CoV S protein, particularly in solution binding, but the residues required for contact are similar. We also confirm that the ACE-2-binding site of NL63 S lies between residues 190 and 739. A lower-affinity interaction with ACE-2 might partly explain the different pathological consequences of infection by SARS-CoV and NL63.</div>
</front>
</TEI>
<affiliations><list><country><li>Pays-Bas</li>
<li>Royaume-Uni</li>
</country>
<region><li>Hollande-Septentrionale</li>
</region>
<settlement><li>Amsterdam</li>
</settlement>
<orgName><li>Université d'Amsterdam</li>
</orgName>
</list>
<tree><country name="Royaume-Uni"><noRegion><name sortKey="Mathewson, Alison C" sort="Mathewson, Alison C" uniqKey="Mathewson A" first="Alison C." last="Mathewson">Alison C. Mathewson</name>
</noRegion>
<name sortKey="Bishop, Alexandra" sort="Bishop, Alexandra" uniqKey="Bishop A" first="Alexandra" last="Bishop">Alexandra Bishop</name>
<name sortKey="Hongying Chen" sort="Hongying Chen" uniqKey="Hongying Chen" last="Hongying Chen">HONGYING CHEN</name>
<name sortKey="Jones, Ian M" sort="Jones, Ian M" uniqKey="Jones I" first="Ian M." last="Jones">Ian M. Jones</name>
<name sortKey="Junyuan Ren" sort="Junyuan Ren" uniqKey="Junyuan Ren" last="Junyuan Ren">JUNYUAN REN</name>
<name sortKey="Kemp, Fred" sort="Kemp, Fred" uniqKey="Kemp F" first="Fred" last="Kemp">Fred Kemp</name>
<name sortKey="Xiaodong Xu" sort="Xiaodong Xu" uniqKey="Xiaodong Xu" last="Xiaodong Xu">XIAODONG XU</name>
<name sortKey="Yongxiu Yao" sort="Yongxiu Yao" uniqKey="Yongxiu Yao" last="Yongxiu Yao">YONGXIU YAO</name>
</country>
<country name="Pays-Bas"><region name="Hollande-Septentrionale"><name sortKey="Berkhout, Ben" sort="Berkhout, Ben" uniqKey="Berkhout B" first="Ben" last="Berkhout">Ben Berkhout</name>
</region>
<name sortKey="Van Der Hoek, Lia" sort="Van Der Hoek, Lia" uniqKey="Van Der Hoek L" first="Lia" last="Van Der Hoek">Lia Van Der Hoek</name>
</country>
</tree>
</affiliations>
</record>

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Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2

Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2

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